Knocking down gene function with an RNA aptamer expressed as part of an intron
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منابع مشابه
Knocking down gene function with an RNA aptamer expressed as part of an intron
We developed a powerful expression system to produce aptamers and other types of functional RNA in yeast to examine their effects. Utilizing the intron homing process, the aptamer-coding sequences were integrated into hundreds of rRNA genes, and the aptamers were transcribed at high levels by RNA polymerase I without any additional promoter being introduced into the cell. We used this system to...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2010
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkq529